Serosurvey of Canine Dirofilariasis in a Population of Southern Guatemala

Main Article Content

Marieandrée Arimany


Introduction: Canine dirofilariasis, is a vector-borne disease caused by the parasitic filarial nematode named Dirofilaria immitis. Cardiopulmonary dirofilariasis is a worldwide distributed disease affecting domestic and wild canines as well as felines, causing pulmonary or cutaneous infections in humans. In recent years, scientists have reported the importance of investigating human cases of dirofilariasis since it can be easily confused with lung disease. Guatemala has a high-density of the canine population. Few studies have reported a high prevalence of canine dirofilariasis; nevertheless, knowledge in Guatemala of Dirofilaria immitis is scarce and practically inexistent. Given this, the current study aimed to explore the circulation of antibodies against D. immitis in dogs in the southern part of Guatemala.

Materials and methods: Data were recollected from 110 male and female dogs aged more than a year from various breeds in the village “El Brito”, Escuintla, Guatemala. One milliliter of blood was collected from each dog. The samples were analyzed using ELISA test to examine the presence of antibodies in adult worms.

Results: The prevalence determined for antibodies against Dirofilaria immitis of sampled canines was 2.7%, and the most frequent age of the canines was 1-3 years old (85%).

Conclusions: According to the obtained data, the prevalence of canine dirofilariasis in a southern region of Guatemala was low.

Article Details

How to Cite
Arimany, M. (2022). Serosurvey of Canine Dirofilariasis in a Population of Southern Guatemala. Journal of Veterinary Physiology and Pathology, 1(4), 69–73.
Original Articles


Adebayo OO, Akande FA, and Adenubi OT. Canine dirofilariasis: A case report and review of the literature. J Folia Veterinaria. 2020; 64(3): 75-81. DOI:

Anvari D, Narouei E, Daryani A, Sarvi S, Moosazadeh M, Hezarjaribi H, et al. The global status of Dirofilaria immitis in dogs: A systematic review and meta-analysis based on published articles. Res Vet Sci. 2020; 131: 104-116. DOI:

Wang D, Bowman DD, Brown HE, Harrington LC, Kaufman PE, McKay T, et al. Factors influencing U.S. canine heartworm (Dirofilaria immitis) prevalence. Parasites Vectors. 2014; 7: 264. Available at:

Dearsley EJ, O'Handley RM, and Caraguel C. Is canine heartworm (Dirofilaria immitis) endemic to South Australia? J Aust Vet Journal. 2019; 97(6): 191-196. DOI:

Otranto D and Deplazes P. Zoonotic nematodes of wild carnivores. Int J Parasitol: Parasites and Wildlife. 2019; 9: 370-383. DOI:

Penezić A, Selaković S, Pavlović I, and Ćirović D. First findings and prevalence of adult heartworms (Dirofilaria immitis) in wild carnivores from Serbia. Parasitol Res. 2014; 113: 3281-3285. DOI:

Ionică AM, Matei IA, D’Amico G, Ababii J, Daskalaki AA, Sándor AD, et al. Filarioid infections in wild carnivores: A multispecies survey

in Romania. Parasites Vectors. 2017; 10: 332. DOI:

Carretón E, Morchón R, and Montoya-Alonso JA. Cardiopulmonary and inflammatory biomarkers in heartworm disease. Parasites Vectors. 2017; 10: 534. DOI:

Stone M, Dalal I, Stone C, and Dalal B. Uptake in Pulmonary Dirofilariosis. J Radiol Case Rep. 2015; 9: 28-33. DOI:

Tumolskaya NI, Pozio E, Rakova VM, Supriaga VG, Sergiev VP, Morozov EN, et al. Dirofilaria immitis in a child from the

Russian Federation. J Parasite. 2016; 23: 37. DOI:

Simón F, Siles-Lucas M, Morchón R, González-Miguel J, Mellado I, Carretón E, et al. Human and animal dirofilariasis: The emergence of a zoonotic mosaic. Cli Microbiol Rev. 2012; 25: 507-544. DOI:

Wei L, Kelly P, Ackerson K, Sayed H, Kaltenboeck B, and Wang C. Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys, and Ehrlichia canis in dogs on Costa Rica. Acta Parasitol. 2014; 60: 21-25. DOI:

Maggi RG and Krämer F. A review on the occurrence of companion vector-borne diseases in pet animals in Latin America. Parasites Vectors. 2019; 12: 145. DOI:

Grech S. [Internet] Disability, poverty, and education: Perceived barriers and (dis)connections in rural Guatemala. Disability and the Global South. 2014; 1: 128-152. Available at:

Bader J, Ramos RAN, Otranto D, and Dantas-Torres F. Vector-borne pathogens in dogs from Guatemala, Central America. Vet

Parasitol: Reg Studies Rep, 2020; 22: 100468. DOI:

INSIVUMEH. [Internet] Datos Metorologicos de los Departamentos. Instituto Nacional de Sismología, Vulcanología, Meteorología e Hidrología. 2015 [cited 2022 August 30] Available at:

Segeplan. [Internet] Plan de Desarrollo Guanagazapa Escuintla. Secretaría de Planificación y Programación de la Presidencia. 2010; Available at:

SIGSA. [Internet] Lineamientos técnicos para la campaña nacional de vacunación antirrábica canina, Guatemala. Ministerio de

salud pública y asistencia social. 2017; Available at:

Bamorovat M, Sharifi I, Fasihi M, Nasibi S, Sadeghi B, Khedri J, et al. [Internet] Parasitological, serological, and molecular study of Dirofilaria immitis in domestic dogs, Southeastern Iran. Iran

J Parasitol. 2017; 12(2): 260-266. Available at:

Vezzani D, Carbajo AE, Fontanarrosa MF, Scodellaro CF, Basabe J, Cangiano G, et al. Epidemiology of canine heartworm in its southern distribution limit in South America: Risk factors, interannual trend and spatial patterns. Vet Parasitol. 2011; 176(2-3): 240-249. DOI:

Ahmad y. Veterinary Parasitology. In: Taylor M, Coop RL, and Wall RL, editors. 3rd ed. Oxford: Blackwell; 2007. Available at:

Johansson S. [Internet] General health conditions in the dog population of Lilongwe. Uppsala: SLU, Dept. of Clinical Sciences. 2016 [cited 2022 September 3] Available at: nbn:se:slu:epsilon-s-5807

Romero-Rodríguez P, García-y-González E, Santos-Sotomaior C, Pineda-Burgos B, Olivar-Valladolid G, Hernández-Ruiz P, et al. Prevalencia de Dirofilaria immitis en caninos domésticos de dos municipios del trópico de Guerrero, México. J Aban Vet. 2019; 9: 1-11. DOI:

McCown ME, Monterroso VH, and Cardona W. Surveillance for Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, and Dirofilaria immitis in dogs from three cities in Columbia. J CES

Med Vet y Zootecnia. 2015; 10(2): 224-231. Available at:

Labarthe N, Pereira MC, Barbarini O, McKee W, Coimbra CA, and Hoskins J. [Internet] Serologic prevalence of Dirofilaria immitis, Ehrlichia canis, and Borrelia burgdorferi infections in Brazil. Vet therap: Res Appl Vet Med. 2003; 4(1): 67-75. Available at:

Ramos DG, Zocco BKA, Torres M, Braga IA, Pacheco R, and Sinkoc AL. Helminths parasites of stray dogs (Canis lupus familiaris) from Cuibá, Midwestern of Brazil. Universida de Estadual de Londrina. 2015; 36(2): 889-893. DOI: 0359.2015v36n2p889

Movilla R, García C, Siebert S, and Roura X. Countrywide serological evaluation of canine prevalence for Anaplasma spp., Borrelia burgorferi (sensu lato), Dirofilaria immitis and Ehrlichia canis

in Mexico. Parasites Vectors. 2016; 9: 421. DOI:

Taylor MJ, Voronin D, Johnston KL, and Ford L. Wolbachia filarial interactions. Cell Microbiol. 2012; 15(4): 520-526. DOI:

Bazzocchi C, Mortarino M, Grandi G, Kramer LH, Genchi C, Bandi C, et al. Combined ivermectin and doxycycline treatment has microfilaricidal and adulticidal activity against Dirofilaria immitis in experimentally infected dogs. Int J Parasitol. 2008; 38(12): 1401-1410. DOI:

Kramer I, Crosara S, Gnudy G, Genchi M, Mangia C, Viglietti A, et al. Wolbachia, doxycycline and macrocyclic lactones: New prospects in the treatment of canine heartworm disease. Vet Parasitol. 2018; 254: 95-97. DOI:

American heartworm society. [Internet] Prevention, diagnosis, and management of heartworm (Dirofilaria immitis) infection

in dogs. 2018 [cited 2022 September 5] Available at:

Spence M R, Federico JL, and Reiskind MH. Mosquito diversity and dog heartworm prevalence in suburban areas. Parasites Vectors. 2020; 13(1): 12. DOI:

Izquierdo A, Boucourt E, Jiménez M, and Carrera M. Actualización clínica-epidemiológica: Infección humana por dirofilaria immitis y otras filarias zoonóticas. J Sci Res. 2019; 4(3): 1-17. DOI: