Transmission Dynamics of Cryptosporidium in Calves and Children from Southwestern Ethiopia

Main Article Content

Sadik Zakir Abadura
Wubit Tafese
Abdu Mohamed
Suresh Kumar Pnair


Introduction: Cryptosporidium is a protozoan parasite that can affect both humans and animals. The present study aimed to determine the prevalence and risk factors of cryptosporidiosis in bovine calves and children in Jimma, southwestern Ethiopia. This cross-sectional study was conducted from December 2019 to July 2020 to assess the prevalence and risk factors of the infection among calves younger than 1 year and children younger than 5 years.

Materials and methods: Fecal samples were collected from 384 calves and 147 children and examined by the Modified Ziehl-Neelson staining method.

Results: The overall prevalence was 8.1% in calves and 7.5% in children. The multivariable logistic regression analysis showed that the risk of Cryptosporidiosis was significantly higher in younger calves < 3 months followed by river water users and calves kept in dirty pens. Moreover, the analysis of children data indicated that the risk of Cryptosporidiosis was significantly higher in children >1 year, followed by children settled around the rural area, children whose families had a poor habit of handwashing after attending cattle, children whose their family had a high level of contact with calves, and children who were drinking river water.

Conclusion: The present study revealed that the high prevalence of cryptosporidiosis may be due to poor hygienic status, unclean sources of water, attending farms, and contact with calves or their feces. Generally, poor personal and dairy farm hygiene and drinking river water source were the factors contributing to the disease.

Article Details

How to Cite
Zakir Abadura, S., Tafese, W., Mohamed, A., & Pnair, S. K. (2022). Transmission Dynamics of Cryptosporidium in Calves and Children from Southwestern Ethiopia. Journal of Veterinary Physiology and Pathology, 1(1), 26–36.
Original Articles


Chalmers RM, and Giles M. Zoonotic Cryptosporidiosis in the UK-challenges for control. Appl Microbiol. 2010; 109: 1487-1497. DOI:

Gharpure R, Perez A, Miller AD, Wikswo ME, Silver R, and Hlavsa MC. Cryptosporidiosis Outbreaks -the United States, 2009-2017. MMWR Morb Mortal Wkly Rep. 2019; 68: 568-572. DOI:

Dillingham RA, Lima AA, and Guerrant RL. Cryptosporidiosis: Epidemiology and impact. Microbes Infect. 2002; 4: 1059-1066. DOI:

Tzipori S, and Widmer G. A hundred-year retrospective on cryptosporidiosis. Trends Parasitol. 2008; 24: 184-189. DOI:

Navin TR, and Juranek DD. Cryptosporidiosis: Clinical, epidemiologic, and parasitologic review. Rev Infect Dis. 1984; 6: 313-327. DOI:

Shirley DA, Moonah SN, and Kotloff KL. The burden of disease from Cryptosporidiosis. Curr Opin Infect Dis. 2012; 25: 555-563. DOI:

Warren C, and Guerrant R. Clinical disease and pathology. In: Fayer R, Xiao L, editors. Cryptosporidium and Cryptosporidiosis. 2ed.

Boca Raton, FL: CDC Press, 2008; 1: 235-254. DOI:

Liu L, Johnson HL, Cousens S, Perin J, Scott S, Lawn JE, Rudan I, et al. Child health epidemiology reference group of WHO and UNICEF Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000. Lancet. 2012; 379: 2151-2156. DOI:

Mumtaz S, Ahmed J, and Ali L. Frequency of Cryptosporidium infection in children under five years of age having diarrhea in the North West of Pakistan. Afr J Biotechnol. 2010; 9: 1230-1235. DOI:

Pumipuntu N, and Piratae S. Cryptosporidiosis: A zoonotic

disease concern, Vet World, 2018; 11(5): 681-686. DOI:

Painter JE, Hlavsa MC, Collier SA, Xiao L, and Yoder JS. Cryptosporidiosis Surveillance United States, 2011-2012. MMWR Surveill Summ. 2015; 64: 1-13. DOI:

Bouzid M, Hunter PR, Chalmers RM, and Tyler KM. Cryptosporidium pathogenicity and virulence. Clin Microbiol Rev. 2013; 26: 115-134. DOI:

Weber R, Bryan RT, and Juranek DD. Improved stool concentration procedure for detection of Cryptosporidium oocysts in fecal specimens. J Clin Microbiol. 1992; 30(11): 2869-2873. DOI:

Thomson S, Hamilton CA, Hope JC, Katzer F, Mabbott NA, Morrison LJ, and Innes EA. Bovine Cryptosporidiosis: Impact, host-parasite interaction, and control strategies Vet Res. 2017; 48(1): 42. DOI:

Pumipuntu N, and Piratae S. Cryptosporidiosis: A zoonotic

disease concern. Vet World. 2018; 11(5): 681-686. DOI:

Umer HA, Lawal IA, Okubanjo O, and Wakawa AM. Morphometric identification, gross and histopathological leisions of Eimeria species in Japanese quailsn (Coturnix coturnix japonica in Zaria, Nigeria. J Vet Med. 2014. DOI:

Somchal J, Rattana T, SutinY, and Nutaros C. Simple method for long-term cryopreservation of Cryptosporidium oocysts for morphometric and molecular analysis. Trop Med Int Health. 2002; 7(3): 257-264. DOI:

Abebe R, Wossene A, and Kumsa B. An epidemiological study of Cryptosporidium infection in dairy calves on selected dairy farms of central Ethiopia. Revue Med Vet. 2008; 159(2): 107. Available at:

Ayalew D., Boelee E., Endeshaw T, and Petros B. Cryptosporidium and Giardia infection and drinking water sources among children in Lege Dini, Ethiopia. Trop Med Intern Heal. 2008; 13: 472-475. DOI:

Regassa A, Gizaw O, Abunna F, Abebe R, Beyene D, Megersa B, Debela E, et al. Cryptosporidium in Calves, Lambs and Kids in Haramaya, Eastern Ethiopia. Ethiop Vet. 2013; 17(1): 81-94. DOI:

Adamu H, Petros B, Zhang G, Kassa H, Amer S, Ye J, Feng Y, et al. Distribution and clinical manifestations of Cryptosporidium species and subtypes in HIV/AIDS patients in Ethiopia. PLoS Negl Trop Dis. 2014; 8: e0002831. DOI:

Kibru G, and Mekete G. Prevalence of Cryptosporidium infection at the pediatrics clinic of jimma hospital, Southwest Ethiopia. Ethiopian

J of Health Scie. 2000; 10(2): 123-127. Available at:

Manyazewal A. Prevalence and molecular characterization of Cryptosporidium species in humans and cattle in Addis Ababa and its environs, Ethiopia .2017; PhD dissertation Addis Ababa University. DOI:

Thrusfield M. Veterinary Epidemiology. 3rd ed. Blackwell Sci. Ltd. Royal School of Veterinary Studies, University of Edinburgh, UK. 2007; 47: 1117. Available at:


Megersa M, Feyisa A, Wondimu A, and Jibat T. Herd composition and characteristics of dairy production in Bishoftu Town, Ethiopia.

J Agric Ext Rural Dev. 2011; 3(6): 113-117. Available at:


Ehsan AM, Geurden T, Casaert S, Parvin SM, Islam TM, Ahmed UM, Levecke B, et al. Assessment of zoonotic transmission of Giardia

and Cryptosporidium between cattle and humans in rural

villages in Bangladesh. PloS One. 2015; 10(2): e0118239. DOI:

Wegayehu T, Adamu H, and Petros B. Prevalence of Giardia duodenalis and Cryptosporidium species infections among children and cattle in North Shewa Zone, Ethiopia. BMC Infect Dis. 2013; 13(1): 419. DOI:

Adamu H, Petros B, Zhang G, Kassa H, Amer S, Ye J, Feng Y, et al. Distribution and clinical manifestations of Cryptosporidium species and subtypes in HIV/AIDS patients in Ethiopia. PLoS Negl Trop Dis. 2014; 8(4): 2831. DOI:

Alemayehu R, Oda G, Fufa A, Rahmeto A, Desta B, Bekele M, Etana D, et al. Cryptosporidium in calves, lambs and kids at Haramaya,

Eastern Ethiopia. Ethiopian Vet J. 2013; 17(1): 81-94. DOI:

Ayele A, Seyoum Z, and Leta S. Cryptosporidium infection in bovine calves: prevalence and potential risk factors in northwest Ethiopia. BMC Res Not. 2018; 11(1): 1-6. DOI:

Fikre B, L. Diriba E, Birhanu A, and Ayisha A. Prevalence and risk factors of Cryptosporidiosis in dairy calves in Asella Town, South Eastern, Ethiopia. APG, 2017; 8(1): 50-57. Available at:

Korpe PS, Haque R, Gilchrist C, Valencia C, Niu F, Lu M, Ma JZ, et al. Natural history of Cryptosporidiosis in a longitudinal study of slum-dwelling Bangladeshi children: Association with severe malnutrition. PLoS Negl Trop Dis. 2016; 10(5): e0004564. DOI:

Matthys B, Bobieva M, Karimova G, Mengliboeva Z, Jean-Richard V, Hoimnazarova M, Kurbonova M, et al. Prevalence and risk factors of helminths and intestinal protozoa infections among children from primary schools in western Tajikistan. Parasites and Vect. 2011; 4(1): 195. DOI:

Garcia JC, French N, Pita A, Velathanthiri N, Shrestha R, and Hayman D. Local and global genetic diversity of protozoan parasites: Spatial distribution of Cryptosporidium and Giardia genotypes. PLoS Negl Trop Dis. 2017; 11(7): e0005736. DOI:

Ketema A, Tafese W, and Yadeta W. Prevalence of Cryptosporidiosis and its associated risk factors in calves kept in different management system in haramaya district, Eastern Ethiopia. European Bio Scie. 2019; 11 (4): 127-133. DOI:

Santın M, Trout JM, Xiao L, Zhou L, Greiner E, and Fayer R. Prevalence and age-related variation of Cryptosporidium species and genotypes

in dairy calves. Vet Parasitol. 2004; 122(2): 103-117. DOI:

Azami M. Prevalence of Cryptosporidium infection in cattle in Isfahan, Iran. J of Eukaryotic Microbiol. 2007; 54(1): 100-102. DOI:

Nguyen ST, Nguyen DT, Le DQ, Le Hua LN, Van Nguyen T, Honma H, and Nakai Y. Prevalence and first genetic identification of Cryptosporidium spp. in cattle in central Viet nam. Vet Parasitol. 2007; 150(4): 357-361. DOI:

Inpankaew T, Jiyipong T, Wongpanit K, Pinyopanuwat N, Chimnoi W, Kengradomkij C, Xuan X, et al. Molecular detection of Cryptosporidium spp. infections in water buffaloes from northeast Thailand. Trop animal health and Produc. 2014; 46(2): 487-490. DOI:

Becker DJ, Oloya J, and Ezeamama AE. Household socioeconomic and demographic correlates of Cryptosporidium seropositivity in the United States. PLoS Negl Trop Dis. 2015; 9(9): e0004080. DOI:

Swai ES, French NP, Karimuribo ED, Fitzpatrick MJ, Bryant DM, Kambarage, and Ogden NH. Prevalence and determinants of Cryptosporidium Spp. in small holder dairy cattle in Iringa and Tanga Regions of Tanzania. Onderst. Vet Res. 2007; 74: 23-29. DOI:

Trotz-Williams LA, Martin SW, Leslie KE, Duffield T, Nydam DV, and Peregrine AS. Calf level risk factors for neonatal diarrhea and shedding of Cryptosporidium parvum in Ontario dairy calves. Prev Vet Med. 2007; 82: 12-28. DOI:

Causape AC, Quılez J, Sánchez-Acedo C, Del Cacho E, and López-Bernad F. Prevalence and analysis of potential risk factors for Cryptosporidium parvum infection in lambs in Zaragoza (Northeastern Spain). Vet Parasitol. 2002; 104(4): 287-298. DOI:

Yang W, Chen P, Villegas EN, Landy RB, Kanetsky C, Cama V, Dearen T, et al. Cryptosporidium source tracking in the Potomac River watershed. Appl Environ Microbiol. 2008; 74(21): 6495-6504. DOI:

Kvac M, Němejc K, Kestřánová M, Květoňová D, Wagnerová P, Kotková M, Rost M, et al. Age related susceptibility of pigs to Cryptosporidium scrofarum infection. Vet Parasitol. 2014; 202: 330-334. DOI:

Sherchand SP, Joshi D, Adhikari N, Poudel RC, Pant KP, KC M, Shrestha D, et al. Prevalence of Cryptosporidiosis among school going children in Kathmandu, Nepal. EC Microbiol. 2016; 4(1): 641-646. Available at:

Thivierge K, Iqbal A, Dixon B, Dion R, Levesque B, Cantin P, Cédilotte L, et al. Cryptosporidium hominis is a newly recognized pathogen in the Arctic region of Nunavik, Canada: molecular characterization of an outbreak. PLoS Neglect Tropic Dis. 2016; 10(4): e0004534. DOI:

Bodhidatta L, Wongstitwilairoong B, Khantapura P, Shrestha SK, Wangchuk S, and Raj PA. Crypto: Prevalence in children in Nepal

and Bhutan. Vet Med Res. 2016; 3: 1059. Available at:

Liu H, Shen Y, Yin J, Yuan Z, Jiang Y, Xu Y, Pan W, et al. Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia, and Cyclospora in diarrheal outpatients in China. BMC Infect Dis. 2014; 14(1): 1-6. DOI:

Gatei W, Wamae CN, Mbae C, Waruru A, Mulinge E, Waithera T, Gatika SM, et al. Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya. Am J Trop Med Hyg. 2006; 75(1): 78-82. DOI:

Morse TD, Nichols RAB, Grimason AM, Campbell BM, Tembo KC, and Smith HV. Incidence of Cryptosporidiosis species in paediatric patients in Malawi. Epid Infect. 2007; 135(8): 1307-1315. DOI:

Wells B, Shaw H, Hotchkiss E, Gilray J, Ayton R, Green J, Katzer F, et al. Prevalence, species identification and genotyping Cryptosporidium from livestock and deer in a catchment in the Cairngorms with a history of a contaminated public water supply. Parasites Vect. 2015; 8(1): 66. DOI:

Speich B, Croll D, Fürst T, Utzinger J, and Keiser J. Effect of sanitation and water treatment on intestinal protozoa infection: A systematic review and meta-analysis. The Lancet Infect Dis. 2016; 16(1): 87-99. DOI:

Khan A, Shams S, Khan S, Khan MI, Khan S, and Ali A. Evaluation of prevalence and risk factors associated with Cryptosporidium infection in rural population of district Buner, Pakistan. PLoS One. 2019; 14(1): DOI: