_1780291729 20260601052849000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Journal of Veterinary Physiology and Pathology J. Vet. Physiol. Pathol. 2821-0328 12 09 2025 4 4 Phebe Oluwatoyin Okunsaya https://orcid.org/0009-0009-4512-1571 Georgina Ijeoma Ukonu https://orcid.org/0009-0003-7436-9206 Abdulrauf Adekunle Usman https://orcid.org/0009-0007-6614-6343 Theophilus Aghogho Jarikre https://orcid.org/0000-0001-8222-198X Ozomata Daniel Raji https://orcid.org/0009-0006-6584-1558 Introduction: Aflatoxin B1 (AFB1) stands out as one of the most damaging toxins in poultry, causing oxidative stress and immunosuppression in broiler chickens. The present study aimed to investigate the protective effects of vitamins A and E on hematological and biochemical parameters in broiler chickens intoxicated with AFB1.Materials and methods: A total of 96 day-old unsexed broiler chickens, weighing approximately 40 grams, were randomly divided into six groups. Each group consisted of 16 chickens, with two replicates and eight chickens per replicate. The first treatment compromised commercial feed with 35 µg/kg of AFB1 and 10 mg of vitamin A (T1), the second treatment included commercial feed with 35 µg/kg of AFB1 and 15 mg of vitamin A (T2), third treatment included commercial feed with 35 µg/kg of AFB1 and 5 mg of vitamin E (T3), fourth treatment had commercial feed with 35 µg/kg of AFB1 and 10 mg of vitamin E (T4), the negative control had commercial feed with 35 µg/kg of AFB1 without any vitamins (T5), and the positive control group were given commercial feed only (T6). The entire study was conducted over 42 days, and hematological and serum biochemical parameters were assessed on day 42. Results: Differences in hematological and biochemical parameters were not statistically significant across groups. However, T3 had the highest values of packed cell volume (31%), hemoglobin (10.05 g/dl), red blood cell (3.30 × 10⁶/μl), and white blood cell (15.95 × 103/μl). Additionally, in T4, the serum biochemical parameters indicated the lowest values of aspartate aminotransferase (178.50 U/L), alkaline phosphatase (183.00 U/L), and blood urea nitrogen (1.25 mg/dL), numerically compared to the other groups. Conclusion: The present study indicated that vitamins can be used as a strategic dietary ingredient in reducing the effects of aflatoxins. Supplementing with antioxidants such as vitamin E reduces oxidative stress, stabilizes liver and kidney functions, and supports poultry health under AFB1 exposure. 12 29 2025 58 63 https://creativecommons.org/licenses/by/4.0 10.58803/jvpp.v4i4.71 https://jvpp.rovedar.com/index.php/JVPP/article/view/71 https://jvpp.rovedar.com/index.php/JVPP/article/download/71/97 https://jvpp.rovedar.com/index.php/JVPP/article/download/71/97 10.3390/toxins13010046 10.1111/j.1364-3703.2007.00436.x Ravindran V. Poultry feed availability and nutrition in developing countries. Poultry Develop Rev. 2013; 2(11): 60-63. Available at: http://213.55.90.4/admin/home/Dmu%20Academic%20Resource/Agriculture%20And%20Natural%20Resource/postgraduate/animal%20production/Poultry%20farming.pdf#page=66 10.1007/s12011-016-0923-9 10.51791/njap.vi.5051 10.1093/ajcn/80.5.1106 10.1080/713654986 10.3945/ajcn.112.034637 Surai PF. Natural antioxidants in avian nutrition and reproduction. United Kingdom: Nottingham University Press; 2002. p. 464-466. Available at: http://www.feedfood.co.uk/download/Imm_my_2002a.pdf Feldman BV, Zinkl JG, Jain NC, and Schalm OW. Normal avian hematology: Chicken and turkey. In: Feldman BF, Zinkl JG, and Jain NC, editors. Schalm's veterinary hematology. 5th ed. Lippincott Williams & Wilkins, 2000. p. 1147-1154. Available at: https://lccn.loc.gov/99089116 10.3382/ps.2009-00216 10.3389/fanim.2021.775311 Cobb. Cobb broiler management guide. 2021. Available at: https://www.cobbgenetics.com/assets/Cobb-Files/Broiler-Guide_English-2021-min.pdf National research council (NRC). Nutrient requirements of poultry. 9th ed. Subcommittee on poultry nutrition. National research council. National Academies Press, 1994. Available at: https://books.google.com/books?id=bbV1FUqRcM0C&printsec=frontcover&hl=es&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false Poultry farm guide. Poultry vaccination and schedule in Nigeria for layers and broilers. 2025. Available at: https://www.poultryfarmguide.com/blog/poultry-vaccination-and-schedule-in-nigeria-for-layers-and-broilers 10.36380/jwpr.2024.33 10.3390/agriculture8100149 10.4314/gjpas.v30i4.4 Campbell TW. Avian hematology and cytology. 2nd ed. Ames: Iowa State University Press, 1995. Available at: https://lccn.loc.gov/94036354 10.1007/s13765-018-0358-4 10.3389/fvets.2022.993039 Shlig AA. Effect of vitamin E and selenium supplement in reducing aflatoxicosis on performance and blood parameters in broiler chicks. Iraqi J Vet Sci. 2009; 23(1): 97-103. Available at: https://iasj.rdd.edu.iq/journals/uploads/2025/09/11/edea362a5cfd95965dc8324493337d3f.pdf