_1794633044 20261114051044000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Journal of Veterinary Physiology and Pathology J. Vet. Physiol. Pathol. 2821-0328 09 16 2026 5 3 Debashis Dutta https://orcid.org/0009-0002-2421-3863 Subhashish Batabyal https://orcid.org/0000-0002-6888-0222 Apratim Maity https://orcid.org/0000-0001-8498-3558 Mayukh Ghosh https://orcid.org/0000-0001-5381-7901 Introduction: Pesticide residues in feed and water can adversely affect broiler chicken production. This preliminary study aimed to determine the maximum tolerated dose of emamectin benzoate (EB), profenofos, and a sulphur-based product, estimate the profenofos LD50, and evaluate the efficacy of dietary charcoal plus L-carnitine in mitigating the resulting toxicity in broiler chickens.Materials and methods: A total of 240 broiler chickens of both sexes were procured at 10 days of age and divided into a control and three treatment groups. The EB was given in drinking water at 300, 500, 1800, and 2300 mg/kg of body weight (BW), profenofos was administered by oral gavage in eight independent dose groups (2.55, 5, 7.5, 10, 20, 50, 100, and 600 mg/kg BW) for acute lethality; and the sulphur-based product was administered in feed (300-2300 mg/kg). The control group was sampled on day 15. Chickens received the EB and sulphur-based product on days 17 and 19, respectively, and the blood samples were collected on days 18 and 20 from the groups. Serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), creatinine, alongside mortality and clinical signs, were recorded over 7 days. Dietary charcoal plus L-carnitine was then given to surviving broiler chickens in the EB, profenofos, and sulphur-based product groups.Results: Profenofos induced dose-dependent mortality, with an acute oral LD₅₀ of approximately 4.7 mg/kg BW. In contrast, EB produced no mortality or clinical signs up to 2300 mg/kg. Across all four EB groups, ALP levels were elevated compared to the control group, whereas SGPT, SGOT, and serum creatinine remained largely unchanged. However, because chickens in EB groups were sampled at 18-20 days of age, the observed ALP elevation could not be distinguished from age-related physiological changes. In contrast, the sulphur-based product caused no mortality or clinical signs at any dose tested. 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